| 產(chǎn)品名稱(chēng) |
Rosculus sp. |
| 商品貨號(hào) |
B243823 |
| Strain Designations |
BSA-02190017 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
sediment, saltwater Virginia, United States |
| Product Format |
frozen |
| Storage Conditions |
liquid nitrogen vapor phase |
| Type Strain |
no |
| Medium |
ATCC® Medium 1525: Seawater 802 medium
|
| Growth Conditions |
Temperature: 20.0°C Duration: xenic; Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831) and as a food source Protocol: - Scrape the bottom of the flask, quickly agitate and withdraw 0.2 ml of cell suspension.
- Inoculate into 10 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831).
Note: the amoebae settle very quickly, so the transfer must be done very quickly to be effective. - Incubate the flask at 20C.
Interval: biweekly |
| Subcultivation |
Protocol: - Scrape the bottom of the flask, quickly agitate and withdraw 0.2 ml of cell suspension.
- Inoculate into 10 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831).
Note: the amoebae settle very quickly, so the transfer must be done very quickly to be effective. - Incubate the flask at 20C.
Interval: biweekly |
| Cryopreservation |
Storage temperature: liquid nitrogen vapor phase |
| Name of Depositor |
JC Cole, TA Nerad |
| Special Collection |
NSF - Protistology |
| Geographical Isolation |
Cattleshed Marsh (near Hog Island) |
| References |
sampled from a sparina area; Catleshed Marsh (near Hog Island), eastern shore of Virginia
|
